"Party Rock Anthem" singers LMFAO won best group and Hot 100 Song of the Year. Chris Brown won Top R&B Artist and Wiz Khalifa was crowned Top New Artist.
Whitney Houston's daughter Bobbi Kristina accepted the Millennium Award on behalf of her late mother.
"I'm just blessed to have been in such an incredible woman's life," said Bobbi Kristina. "There will never be another one, ever."
John Legend and Jordan Sparks paid tribute in song.
Adam Yaugh of the Beastie Boys and Donna Summer were also remembered.
The show also marked a moment of silence for one of the co-founders of the Bee Gees. Robin Gibb died Sunday after a long battle with cancer.
The Bee Gees was made up of three British-born, Australian-raised brothers. They sold more than 200 million albums and were inducted into the Rock and Roll Hall of Fame. It's reported that Barry Gibb, the last surviving member of the group, held vigil at his brother's bedside in London.
Robin Gibb was 62.